Journal: iScience

Article Title: Actin crosslinking by α-actinin averts viscous dissipation of myosin force transmission in stress fibers

doi: 10.1016/j.isci.2023.106090

Figure Lengend Snippet:

Article Snippet: To examine colocalization of the elastic modulus with SFs, we conducted elasticity mapping by AFM in living cells in which the actin cytoskeleton was labeled with the carboxylated silicon–rhodamine (SiR)-actin live cell imaging probe (Cytoskeleton).

Techniques: Recombinant, Protease Inhibitor, shRNA, Binding Assay, Plasmid Preparation, Software

Journal: PLoS ONE

Article Title: Sustained Ca 2+ mobilizations: A quantitative approach to predict their importance in cell-cell communication and wound healing

doi: 10.1371/journal.pone.0213422

Figure Lengend Snippet: (A) Live-cell imaging of the wound edge of cells preincubated with SiR-Actin Spirochrome (in grayscale) to determine cell migration over a 16 hour period. Scale bar = 66 μm. Traces of the wound area and 8 random cells in the field were drawn over time to observe the rate of cell migration and wound closure for the experimental and control conditions. Colors reflect time and are indicated by time wedge (n = 3). (B) Representative percent wound closure graph of cells preincubated with 10Panx or Panx scrambled peptide control over time (n = 3). (C) Representative cell migration trajectory diagrams of LE and BFLE cells preincubated in either 10Panx or Panx scrambled peptide control. Each line represents the migration path of a single cell plotted from a common origin. Scale bar = 20 μm.

Article Snippet: To examine cell migration and alterations in cell shape, HCLE cells were pre-loaded with either CellMask Deep Red Plasma membrane stain as described above or 1 μM of SiR-Actin Spirochrome probe (Cytoskeleton Inc., Denver, CO) for 10 minutes at 37°C and 5% CO 2 , for imaging of F-actin.

Techniques: Live Cell Imaging, Migration